1x neb t4 ligase buffer Search Results


1x ligase buffer  (New England Biolabs)
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New England Biolabs 1x ligase buffer
1x Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x t4 pnk buffer  (New England Biolabs)
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New England Biolabs 1x t4 pnk buffer
1x T4 Pnk Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x t4 rna ligase buffer  (New England Biolabs)
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New England Biolabs 1x t4 rna ligase buffer
Post-transcriptional processing of lncCOBRA1. (A) Diagram of lncCOBRA1 ( AT1G05913 ) locus. Gray arrows represent the two snoRNAs annotated within lncCOBRA1. Red arrows represent the two primers used for 5′ RACE and red triangle represents the 5′ end identified by 5′ RACE PCR in (B) . Blue arrow represents the primer used for 3′ RACE. Blue triangles represent the 3’ most end identified through Sanger sequencing 14 colonies. (B) Three biological replicates of 5′ RACE with primers indicated in (B) . Red triangles represent the two major bands of PCR product. Ladder is 1 kb + . (C) PCR results from 3′ RACE in Col-0 5-day-old seedlings. <t>–/+T4</t> <t>RNA</t> ligase, –/+SuperScript II. Ladder is 1 kb +.
1x T4 Rna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x t4 rna ligase buffer - by Bioz Stars, 2026-02
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1x t4 ligation buffer  (New England Biolabs)
86
New England Biolabs 1x t4 ligation buffer
Post-transcriptional processing of lncCOBRA1. (A) Diagram of lncCOBRA1 ( AT1G05913 ) locus. Gray arrows represent the two snoRNAs annotated within lncCOBRA1. Red arrows represent the two primers used for 5′ RACE and red triangle represents the 5′ end identified by 5′ RACE PCR in (B) . Blue arrow represents the primer used for 3′ RACE. Blue triangles represent the 3’ most end identified through Sanger sequencing 14 colonies. (B) Three biological replicates of 5′ RACE with primers indicated in (B) . Red triangles represent the two major bands of PCR product. Ladder is 1 kb + . (C) PCR results from 3′ RACE in Col-0 5-day-old seedlings. <t>–/+T4</t> <t>RNA</t> ligase, –/+SuperScript II. Ladder is 1 kb +.
1x T4 Ligation Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t4 dna ligase buffer 1x  (New England Biolabs)
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New England Biolabs t4 dna ligase buffer 1x
Post-transcriptional processing of lncCOBRA1. (A) Diagram of lncCOBRA1 ( AT1G05913 ) locus. Gray arrows represent the two snoRNAs annotated within lncCOBRA1. Red arrows represent the two primers used for 5′ RACE and red triangle represents the 5′ end identified by 5′ RACE PCR in (B) . Blue arrow represents the primer used for 3′ RACE. Blue triangles represent the 3’ most end identified through Sanger sequencing 14 colonies. (B) Three biological replicates of 5′ RACE with primers indicated in (B) . Red triangles represent the two major bands of PCR product. Ladder is 1 kb + . (C) PCR results from 3′ RACE in Col-0 5-day-old seedlings. <t>–/+T4</t> <t>RNA</t> ligase, –/+SuperScript II. Ladder is 1 kb +.
T4 Dna Ligase Buffer 1x, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t4 dna ligase buffer 1x - by Bioz Stars, 2026-02
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1x dnase i reaction buffer  (New England Biolabs)
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New England Biolabs 1x dnase i reaction buffer
Post-transcriptional processing of lncCOBRA1. (A) Diagram of lncCOBRA1 ( AT1G05913 ) locus. Gray arrows represent the two snoRNAs annotated within lncCOBRA1. Red arrows represent the two primers used for 5′ RACE and red triangle represents the 5′ end identified by 5′ RACE PCR in (B) . Blue arrow represents the primer used for 3′ RACE. Blue triangles represent the 3’ most end identified through Sanger sequencing 14 colonies. (B) Three biological replicates of 5′ RACE with primers indicated in (B) . Red triangles represent the two major bands of PCR product. Ladder is 1 kb + . (C) PCR results from 3′ RACE in Col-0 5-day-old seedlings. <t>–/+T4</t> <t>RNA</t> ligase, –/+SuperScript II. Ladder is 1 kb +.
1x Dnase I Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x neb t4 ligase buffer  (New England Biolabs)
96
New England Biolabs 1x neb t4 ligase buffer
Post-transcriptional processing of lncCOBRA1. (A) Diagram of lncCOBRA1 ( AT1G05913 ) locus. Gray arrows represent the two snoRNAs annotated within lncCOBRA1. Red arrows represent the two primers used for 5′ RACE and red triangle represents the 5′ end identified by 5′ RACE PCR in (B) . Blue arrow represents the primer used for 3′ RACE. Blue triangles represent the 3’ most end identified through Sanger sequencing 14 colonies. (B) Three biological replicates of 5′ RACE with primers indicated in (B) . Red triangles represent the two major bands of PCR product. Ladder is 1 kb + . (C) PCR results from 3′ RACE in Col-0 5-day-old seedlings. <t>–/+T4</t> <t>RNA</t> ligase, –/+SuperScript II. Ladder is 1 kb +.
1x Neb T4 Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1x neb t4 ligase buffer/product/New England Biolabs
Average 96 stars, based on 1 article reviews
1x neb t4 ligase buffer - by Bioz Stars, 2026-02
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t4 ligation buffer 1x  (New England Biolabs)
96
New England Biolabs t4 ligation buffer 1x
Post-transcriptional processing of lncCOBRA1. (A) Diagram of lncCOBRA1 ( AT1G05913 ) locus. Gray arrows represent the two snoRNAs annotated within lncCOBRA1. Red arrows represent the two primers used for 5′ RACE and red triangle represents the 5′ end identified by 5′ RACE PCR in (B) . Blue arrow represents the primer used for 3′ RACE. Blue triangles represent the 3’ most end identified through Sanger sequencing 14 colonies. (B) Three biological replicates of 5′ RACE with primers indicated in (B) . Red triangles represent the two major bands of PCR product. Ladder is 1 kb + . (C) PCR results from 3′ RACE in Col-0 5-day-old seedlings. <t>–/+T4</t> <t>RNA</t> ligase, –/+SuperScript II. Ladder is 1 kb +.
T4 Ligation Buffer 1x, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 ligation buffer 1x/product/New England Biolabs
Average 96 stars, based on 1 article reviews
t4 ligation buffer 1x - by Bioz Stars, 2026-02
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1x t4 dna ligation buffer  (New England Biolabs)
96
New England Biolabs 1x t4 dna ligation buffer
Post-transcriptional processing of lncCOBRA1. (A) Diagram of lncCOBRA1 ( AT1G05913 ) locus. Gray arrows represent the two snoRNAs annotated within lncCOBRA1. Red arrows represent the two primers used for 5′ RACE and red triangle represents the 5′ end identified by 5′ RACE PCR in (B) . Blue arrow represents the primer used for 3′ RACE. Blue triangles represent the 3’ most end identified through Sanger sequencing 14 colonies. (B) Three biological replicates of 5′ RACE with primers indicated in (B) . Red triangles represent the two major bands of PCR product. Ladder is 1 kb + . (C) PCR results from 3′ RACE in Col-0 5-day-old seedlings. <t>–/+T4</t> <t>RNA</t> ligase, –/+SuperScript II. Ladder is 1 kb +.
1x T4 Dna Ligation Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1x t4 dna ligation buffer/product/New England Biolabs
Average 96 stars, based on 1 article reviews
1x t4 dna ligation buffer - by Bioz Stars, 2026-02
96/100 stars
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Image Search Results


Post-transcriptional processing of lncCOBRA1. (A) Diagram of lncCOBRA1 ( AT1G05913 ) locus. Gray arrows represent the two snoRNAs annotated within lncCOBRA1. Red arrows represent the two primers used for 5′ RACE and red triangle represents the 5′ end identified by 5′ RACE PCR in (B) . Blue arrow represents the primer used for 3′ RACE. Blue triangles represent the 3’ most end identified through Sanger sequencing 14 colonies. (B) Three biological replicates of 5′ RACE with primers indicated in (B) . Red triangles represent the two major bands of PCR product. Ladder is 1 kb + . (C) PCR results from 3′ RACE in Col-0 5-day-old seedlings. –/+T4 RNA ligase, –/+SuperScript II. Ladder is 1 kb +.

Journal: Frontiers in Plant Science

Article Title: A Conserved Long Intergenic Non-coding RNA Containing snoRNA Sequences, lncCOBRA1 , Affects Arabidopsis Germination and Development

doi: 10.3389/fpls.2022.906603

Figure Lengend Snippet: Post-transcriptional processing of lncCOBRA1. (A) Diagram of lncCOBRA1 ( AT1G05913 ) locus. Gray arrows represent the two snoRNAs annotated within lncCOBRA1. Red arrows represent the two primers used for 5′ RACE and red triangle represents the 5′ end identified by 5′ RACE PCR in (B) . Blue arrow represents the primer used for 3′ RACE. Blue triangles represent the 3’ most end identified through Sanger sequencing 14 colonies. (B) Three biological replicates of 5′ RACE with primers indicated in (B) . Red triangles represent the two major bands of PCR product. Ladder is 1 kb + . (C) PCR results from 3′ RACE in Col-0 5-day-old seedlings. –/+T4 RNA ligase, –/+SuperScript II. Ladder is 1 kb +.

Article Snippet: To remove any 5′ m 7 G caps, 500 ng of the SAP-treated RNA was treated with 12.5 units RNA 5’ Pyrophosphohydrolase (RppH; New England BioLabs; Ipswitch, MA, United States) in 1X T4 RNA Ligase Buffer (New England BioLabs; Ipswitch, MA, United States) supplemented with 20 units RNaseOUT (Invitrogen; Carlsbad, CA, United States) in a total reaction volume of 10 μL for 1 h at 37°C and stored at –20°C overnight.

Techniques: Sequencing